doi: 10.3389/fendo.2026.1877238 Received 10 May 2026 Revised 12 July 2026 Accepted 16 July 2026 Published 31 July 2026 Volume 17 - 2026 Edited by Yao-Wu Liu, Xuzhou Medical University, China Reviewed by Yen-Sung Huang, National Defense Medical Center, Taiwan Sara Gamal, Minia University, Egypt Updates Copyright 2026 Sun, Chen, Huang, Chen, He, Wang and Xie
Measurement of Carnitine, TMAO, -butyrobetaine, d9-carnitine, d9-TMAO, and d9--butyrobetaine Sample preparation and quantification for carnitine and TMAO in healthy volunteers Fifty microliters of plasma and 50 L urine samples were extracted with 450 L and 950 L methanol containing 200 ng/mL isotopically labeled internal standards (d3-carnitine and d9-TMAO) respectively, and the extraction was performed using the Geno/Grinder 2010 (SPEX SamplePrep., Metuchen, NJ, USA) at 1000 rpm for 3 min
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